Serum 抗体 (FITC)
This 猪 多克隆 antibody specifically detects Serum in ELISA, IHC (fro), ICC 和 IFA. It exhibits reactivity toward 人.
产品编号 ABIN458188
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Serum 抗体 (FITC) (ABIN458188)
抗原
Serum
适用
人
宿主
猪
克隆类型
多克隆
标记
FITC
应用范围
ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Indirect Immunofluorescence Assay (IFA)
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原理
- Swine anti Human Serum proteins, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Swine antiSerum to Human Serum proteins.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- In immunoelectrophoresis against normal human serum precipitation can be observed of not less than 15 individual protein components. In direct or indirect fluorescent staining techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Pooled whole human serum of healthy donors and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- FITC-coupled purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- Serum
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别名
- Serum Proteins
抗原
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