TTR 抗体
Our Local Distributor
北京 101111
Quick Overview for TTR 抗体 (ABIN458186)
抗原
See all TTR 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- Swine anti Human prealbumin
-
特异性
- Precipitating polyclonal Swine antiSerum to Human transthyretin (prealbumin).
-
交叉反应 (详细)
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
-
产品特性
- In immunoelectrophoresis and double radial immunodiffusion against normal human serum the precipitin line obtained shows a reaction of full identity with the purified immunogen. The product does not react with any other proteins component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of transthyretin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
-
纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
-
免疫原
- Transthyretin is the fastest protein in the immunoelectrophoresis pattern of human serum. It consists of four identical polypeptide chins with no carbohydrate. The molecular weight is 55,000 and the concentration in normal serum ranges from 100 to 400 mug/ml. It can bind thyroxine and retinal and its concentration is reduced in severe liver diseases. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
-
-
应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL human plasma or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
-
缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled swine serum. No foreign proteins added.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
-
-
- TTR (Transthyretin (TTR))
-
别名
- Transthyretin (Prealbumin)
-
UniProt
- P02766
-
途径
- Hormone Transport
抗原
-