Amylase, Salivary 抗体
适用: 人
ID, IEP, RID
宿主: 绵羊
Polyclonal
unconjugated
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- 抗原
- Amylase, Salivary
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适用
- 人
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- 特异性
- The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
- 产品特性
- Precipitating polyclonal sheep antiserum to human salivary amylase
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the anti-serum.
- 免疫原
- Human salivary amylase is an enzyme hydrolyzing the alpha-1,4-glucan links in poly- saccharides. Its molecular weight is 55,000. The immunogen has been isolated from pooled saliva and purified to homogeneity for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of salivary amylase or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human saliva in agar-block immunodiffusion titration.
- 缓冲液
- Delipidated, heat inactivated, lyophilized, stable antiserum.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- 抗原
- Amylase, Salivary
- 别名
- Salivary Amylase
- 背景
- The reactivity of the antiserum is restricted to salivary amylase. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human saliva a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. No reaction is obtained with human plasma
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