LYZ 抗体 (HRP)
Quick Overview for LYZ 抗体 (HRP) (ABIN458157)
抗原
See all LYZ 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal rabbit antiserum to human lysozyme
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
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免疫原
- Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme. Highly purified lysozyme is isolated from pooled milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lysozyme (LYZ) (AA 1-100) antibody
LYZ 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 ICC, FACS, MS 宿主: 小鼠 Monoclonal LZ598-10G9 unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 24-144) antibodyLYZ 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 22-143) antibodyLYZ 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 18-147) antibodyLYZ 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1D8 unconjugated
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应用备注
- In enzyme-immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:6,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
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缓冲液
- Horseradish peroxidase-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
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储存液
- Without preservative
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储存条件
- 4 °C
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- LYZ (Lysozyme (LYZ))
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别名
- Lysozyme
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背景
- The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immuno-diffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum
抗原
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