LALBA 抗体 (HRP)
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北京 101111
Quick Overview for LALBA 抗体 (HRP) (ABIN458151)
抗原
See all LALBA 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human alpha lactalbumin, conjugated with Horseradish peroxidase
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特异性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human alpha-lactalbumin.
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交叉反应 （详细）
- Homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has not been tested for cross-reactivity
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产品特性
- The defined antibody specificity is directed to alpha-lactalbumin as tested against human milk. Its presence can also be demonstrated in udder tissues. When tested in immunoelectrophoresis and double radial immunodiffusion against serum no reaction is obtained. In enzyme-immunocytochemical and immunohistochemical staining for the detection of alpha lactalbumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure alpha lactalbumin in serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Highly purified alpha-lactalbumin isolated from human milk. Alpha-lactalbumin is one of the major proteins in the alpha region after immunoelectrophoresis of human milk. It has been implicated as a component of the enzyme lactose synthetase which catalyzes the formation of lactose from uridine diphosphate galactose and glucose. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lactalbumin, alpha- (LALBA) antibody
LALBA 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 9C3 unconjugated
anti-Lactalbumin, alpha- (LALBA) (AA 24-141) antibodyLALBA 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) (AA 12-142) antibodyLALBA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) antibodyLALBA 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) (AA 61-142) antibodyLALBA 适用: 人 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) antibodyLALBA 适用: 人, Cow ELISA 宿主: 绵羊 Polyclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) (AA 39-68) antibodyLALBA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) (AA 20-142) antibodyLALBA 适用: Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) antibodyLALBA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Lactalbumin, alpha- (LALBA) (AA 33-142) antibodyLALBA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- LALBA (Lactalbumin, alpha- (LALBA))
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别名
- alpha-Lactalbumin
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UniProt
- P00709
抗原
-
