Fibronectin 抗体
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- 抗原 See all Fibronectin 抗体
- Fibronectin
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Fibronectin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoelectrophoresis (IEP)
- 特异性
- The antiserum does not cross react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with cow, cat, dog and monkey have been observed.
- 产品特性
- Precipitating polyclonal rabbit antiserum to human fibronectin
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- 免疫原
- Fibronectin is a glycoprotein with a molecular weight of 440,000 and consists of two identical peptide chains. Its mean concentration in plasma is 330 µ g/ml. Elevated plasma levels of fibronectin are observed in chronic active liver disease, decreased values are found in cases of acute leukaemia, sepsis, severe burns, acute pancreatitis, inflammatory processes, haemorrhagic shock and polytrauma. Fibronectin is also present on cell surfaces. It cab be cross-linked to the fibrin clot by the action of F XIIIA. As formation of the clot occurs approximately 50% of the plasma Fibronectin is lost. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product Fibronectin Primary Antibody
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anti-Fibronectin (AA 1201-1300) antibody
适用: 人, 小鼠, 大鼠, Cow WB, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin antibody (Biotin)适用: 人, 小鼠, 大鼠, Cow, 猴 WB, IHC, IP, ELISA 宿主: 兔 Polyclonal Biotin
anti-Fibronectin (AA 2117-2236) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 355-558) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin antibody适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Fibronectin (AA 313-607) antibody适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 1998-2106) antibody适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin antibody适用: 人, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin antibody适用: 人 WB, IHC, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 2240-2280) antibody适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated
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- 应用备注
- In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of fibronectin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against pooled human amniotic fluid in agar-block immuno-diffusion ti
- 缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- 抗原
- Fibronectin
- Abstract
- Fibronectin 产品
- 别名
- FN antibody, cig antibody, fibronectin antibody, finc antibody, lets antibody, msf antibody, CIG antibody, ED-B antibody, FINC antibody, FNZ antibody, GFND antibody, GFND2 antibody, LETS antibody, MSF antibody, E330027I09 antibody, Fn antibody, Fn-1 antibody, FIBNEC antibody, fn-1 antibody, fibronectin 1 antibody, FN1 antibody, fn1 antibody, Fn1 antibody
- 背景
- The reactivity of the antiserum is restricted to fibronectin. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma, sera and purified fibronectin a single precipitin line is obtained. Testing in electroimmunodiffusion (Laurell) gives a single precipitation line. No precipitation reaction is obtained with other protein in plasma or serum
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