F13A1 抗体
Quick Overview for F13A1 抗体 (ABIN458124)
抗原
See all F13A1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Precipitating polyclonal rabbit antiserum to human coagulation factor XIII-A
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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免疫原
- Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of 80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about 20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of the total blood FXIII activity with a linear relationship between FXIII level and platelet counts. RAHu/FXIII-A reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit (RAHu/FXIII-S) reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after the release of fibrinopeptides A from fibrinogen. Highly purified FXIII-A is used for the immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 107-345) antibody
F13A1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 372-616) antibodyF13A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 140-377) antibodyF13A1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) antibodyF13A1 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3F1 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) antibodyF13A1 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 9E2 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (N-Term) antibodyF13A1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 46-181) antibodyF13A1 适用: 人 WB, ELISA, IHC, IF, FACS, Coat, StM 宿主: 小鼠 Monoclonal F13A1-1448 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 60-94) antibodyF13A1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB57788 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 380-520) antibodyF13A1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 170-204) antibodyF13A1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB57789 unconjugated
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应用备注
- In precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FXIII. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Increase in plasma FXIII has been observed in type IV hyperlipoproteinaemia and in combined hyperlipodaemia. Congenital and acquired FXIII deficiencies have also been reported. In some cases of myeloma, inhibition of FXIII activity by the paraproteins may occur.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate concentrations of the antigen in ag
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum.
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))
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别名
- Coagulation Factor XIII-A
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背景
- The defined antibody reactivity is restricted to Factor XIII and FXIIIa in plasma and to platelet FXIII. As tested at the level of sensitivity of immunoprecipitation techniques a single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with A subunits of FXIII. No reaction is obtained with FXIII-depleted plasma and with B subunits
抗原
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