alpha Fetoprotein 抗体 (FITC)
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北京 101111
Quick Overview for alpha Fetoprotein 抗体 (FITC) (ABIN458119)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human alpha-1 foetoprotein, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human alphafoetoprotein.
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交叉反应 （详细）
- The antiSerum does not cross react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-foetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Purified human alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum, using a proprietary multiple step method. Several foetoproteins have been demonstrated in human foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It cab easily be identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64,000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma. In pooled foetal/newborn serum a concentration of 1 mg/ml has been measured. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-alpha-Fetoprotein (AFP) (AA 500-609) antibody
AFP 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 犬, 猴 WB, IHC, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 399-605) antibodyAFP 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 409-611) antibodyAFP 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人 WB, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal A2 unconjugated
anti-alpha-Fetoprotein (AFP) (C-Term) antibodyAFP 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 19-397) antibodyAFP 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal OTI2F11 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 281-609) antibodyAFP 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 1087CT8-3-5 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 360-609) antibodyAFP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- alpha-Foetoprotein
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UniProt
- P02771
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途径
- C21-Steroid Hormone Metabolic Process
抗原
-
