LYZ 抗体 (AA 5-220)
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北京 101111
Quick Overview for LYZ 抗体 (AA 5-220) (ABIN458107)
抗原
See all LYZ 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-220
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原理
- Rabbit anti Human lysozyme
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特异性
- Precipitating polyclonal Rabbit antiSerum to Human lysozyme.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure lysozyme. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
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纯化方法
- Purified
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免疫原
- Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme. Highly purified recombinant human lysozyme was used as immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lysozyme (LYZ) (AA 1-100) antibody
LYZ 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 ICC, FACS, MS 宿主: 小鼠 Monoclonal LZ598-10G9 unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 小鼠, 大鼠 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 18-147) antibodyLYZ 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 24-144) antibodyLYZ 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibody (FITC)LYZ 适用: 人 ICFC 宿主: 小鼠 Monoclonal LZ598-10G9 FITC
anti-Lysozyme (LYZ) antibody (PE)LYZ 适用: 人 ICFC 宿主: 小鼠 Monoclonal LZ598-10G9 PE
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
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应用备注
- Precipitation assays. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C.
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- LYZ (Lysozyme (LYZ))
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别名
- Lysozyme
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UniProt
- P61626
抗原
-
