beta-2 Microglobulin 抗体
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北京 101111
Quick Overview for beta-2 Microglobulin 抗体 (ABIN458094)
抗原
See all beta-2 Microglobulin (B2M) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human beta-2 microglobulin
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特异性
- Precipitating polyclonal Rabbit antiSerum to Human beta-2 microglobulin.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail
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产品特性
- The defined antibody reactivity is restricted to beta-2 microglobulin as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained with foetal serum, colostrum and selected pathological plasma and urine containing high levels of beta-2 microglobulin in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of beta-2 microglobulin and to determine its concentration. The presence of non-precipitating antibodies has not been assayed. It does not exclude the use of the antiserum in non-precipitating techniques if proper controls are included.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- Beta-2 microglobulin is a member of the Immunoglobulin superfamily. The amino acid sequence resembles that of immunoglobulin constant domains. It is isolated from urine of patients with impaired readsorption in the kidney tubules. High levels of beta-2 microglobulin are as present in cord blood serum and foetal serum. The host animal is immunised with highly purified beta-2 microglobulin. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-beta-2-Microglobulin (B2M) (AA 21-100) antibody
B2M 适用: 人 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 1-119) antibodyB2M 适用: 人 WB, IF, ELISA, IHC (p), RNAi, PLA 宿主: 小鼠 Monoclonal 3F9-2C2 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人 WB, FACS, ELISA, ICC, IP, IHC (p), RIA 宿主: 小鼠 Monoclonal B2M-01 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal B2M-961 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal B2M-1118 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal rB2M-961 unconjugated Recombinant Antibody
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M 适用: 小鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal A2 unconjugated
anti-beta-2-Microglobulin (B2M) antibody (Biotin)B2M 适用: 人 WB, FACS, ELISA, ICC, IP, IHC (p) 宿主: 小鼠 Monoclonal B2M-01 Biotin
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M 适用: 人 WB, IHC, FACS, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL serum, plasma or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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别名
- beta-2 Microglobulin
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UniProt
- P61769
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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