PROC 抗体
Quick Overview for PROC 抗体 (ABIN458051)
抗原
See all PROC 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Precipitating polyclonal goat antiserum to human protein C
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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免疫原
- Protein C is a glycoprotein (MW 60,000) which is synthesized in the liver. It acts as a trypsin like serine protease but functions in a manner different from that of antithrombin III. Activated protein C (pCa) results from proteolytic cleavage of protein C by thrombin and with protein S (pS) as a cofactor it cleaves factors Va and VIIIa, producing anti-coagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system by inactivating the inhibitor of tissue plasminogen activator (TPA), an enzyme that converts plasminogen into plasmin which cleaves fibrin into fibrin split products. Protein C is consumed during coagulation in vivo. Very little protein C is activated during coagulation in vitro, because this requires the presence of thrombomodulin, a thrombin receptor on endothelial cells. The coagulant activity of protein C is species specific and pCa is specifically regulated by protein C inhibitor (pCI) in the presence of heparin. Normal human plasma contains an average of 0.3 mg/ml. The normal newborn has a protein C level that is 30 to 40% of the normal adult level. Numerous cases with a genetical protein C deficiency associated with thrombotic disease have been reported, frequently with an extensive family history of thrombosis. Patients with a partial protein C deficiency are subject to severe, recurrent deep venous thrombosis and pulmonary embolism. The plasma protein C level should be determined before beginning anticoagulation therapy. Total protein C deficiency in the newborn leads to purpurea fulminans, the diagnosis may be confirmed by determining the protein C levels in both heterozygous parents. Acquired protein C deficiency may result of treatment with oral anticoagulants and of various liver diseases. Plasma protein C levels may also decrease after intravascular coagulation. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Vitamin K-dependent protein C (PROC) (AA 32-131) antibody
PROC 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-Vitamin K-dependent protein C (PROC) antibodyPROC 适用: 人 ELISA, IHC, IHC (p), IEP 宿主: 山羊 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) (AA 446-461), (C-Term) antibodyPROC 适用: 人 WB, IHC, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) (AA 1-236) antibodyPROC 适用: 人 WB, IHC, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) antibodyPROC 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) (Middle Region) antibodyPROC 适用: 人, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) (AA 197-436) antibodyPROC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) (AA 17-166) antibodyPROC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) (AA 288-409) antibodyPROC 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vitamin K-dependent protein C (PROC) antibodyPROC 适用: 人 ELISA, Purif, BP 宿主: 小鼠 Monoclonal HPC-4 unconjugated Recombinant Antibody
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应用备注
- In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion, electro-immunodiffusion (Laurell) immunonephelometry, solid phase and neutralization assays. As catching or detection antibody in ELISA. To prepare an adsorbent for immunoaffinity purification of protein C. Plasma samples and all assay components must contain EDTA to stabilize the proteins. For electroimmunodiffusion a concentration of 1-2% of antiserum in the gel is recommended.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal human plasma in agar-block immunodiffusion titrat
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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储存液
- Without preservative
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储存条件
- 4 °C
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- PROC (Vitamin K-dependent protein C (PROC))
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别名
- Protein C
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背景
- The defined antibody specificity is to human protein C as tested at the level of sensitivity of immuno-precipitation and ELISA techniques. Precipitation obtained in gel immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using various antiserum concentrations against fresh plasma and serum shows a reaction of identity with that obtained with the purified protein C. No reaction is obtained with any other plasma or serum protein component. Complexes of protein C with protein S also react with the antiserum
抗原
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