Lactoferrin 抗体 (HRP)
-
- 抗原 See all Lactoferrin (LTF) 抗体
- Lactoferrin (LTF) (Lactotransferrin (LTF))
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This Lactoferrin antibody is conjugated to HRP
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.
- 产品特性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human lactoferrin
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized fractions of human serum and lactoferrin-depleted human milk as required, to eliminate antibodies reacting with other human serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product LTF Primary Antibody
-
anti-Lactotransferrin (LTF) antibody
LTF 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 25-352) antibodyLTF 适用: 山羊 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) antibodyLTF 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB22673 unconjugated
anti-Lactotransferrin (LTF) (AA 219-248) antibodyLTF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB11921 unconjugated
anti-Lactotransferrin (LTF) (AA 500-710) antibodyLTF 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 63-193) antibodyLTF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 28-351) antibodyLTF 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) antibodyLTF 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 25-352) antibodyLTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) antibodyLTF 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- In enzyme-immunocytochemical and immunohistochemical techniques for the detection of lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:7,000.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
- 缓冲液
- Horseradish peroxidase-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
- 储存液
- Without preservative
- 储存条件
- 4 °C
-
- 抗原
- Lactoferrin (LTF) (Lactotransferrin (LTF))
- 别名
- Lactoferrin (LTF 产品)
- 别名
- LTF antibody, GIG12 antibody, HLF2 antibody, LF antibody, Lf antibody, Csp82 antibody, MMS10R antibody, Ms10r antibody, PLF antibody, TFEW antibody, conalbumin antibody, lactotransferrin antibody, transferrin antibody, transferrin (ovotransferrin) antibody, LTF antibody, CpipJ_CPIJ000114 antibody, TF antibody, Ltf antibody
- 背景
- Tested in immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma
- 途径
- Transition Metal Ion Homeostasis
-
