Kininogen (HMW) 抗体 (Light Chain)
The 山羊 多克隆 anti-Kininogen (HMW) antibody is suitable to detect Kininogen (HMW) in samples from 人. It has been validated for T 和 AGE.
产品编号 ABIN458042
发货至:
中国
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Quick Overview for Kininogen (HMW) 抗体 (Light Chain) (ABIN458042)
抗原
See all Kininogen (HMW) 抗体
Kininogen (HMW)
(Kininogen (High Molecular Weight))
适用
人
宿主
山羊
克隆类型
多克隆
标记
This Kininogen (HMW) antibody is un-conjugated
应用范围
Test (T), Agarose Gel Electrophoresis (AGE)
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抗原表位
- Light Chain
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原理
- Goat anti Human high molecular weight kininogen (light chain specific)
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特异性
- Precipitating polyclonal Goat antiSerum to Human high molecular weight kininogen
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交叉反应 （详细）
- The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Monkey has been observed.
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产品特性
- The defined antibody specificity is to human high molecular weight kininogen. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony, Mancini), using various antiserum concentrations against normal plasma, one single precipitin line is obtained. No precipitation is observed against plasma of patients with high molecular weight kininogen deficiency, but its presence can be demonstrated in normal serum. In double radial immunodiffusion the precipitin line obtained against normal plasma shows a reaction of full identity with the precipitin line with purified high molecular weight kininogen. As precipitating antiserum in electroimmunodiffusion, immunoelectrophoresis and radial immunodiffusion. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- Highly purified molecular weight kininogen isolated from pooled plasma. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Precipitation assays. In electroimmunodiffusion (Laurell) the amount of antiserum required in the agarose gel is usually between 1 and 2 % , depending on the test arrangement.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- Kininogen (HMW) (Kininogen (High Molecular Weight))
抗原
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