Lactoferrin 抗体 (FITC)
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北京 101111
Quick Overview for Lactoferrin 抗体 (FITC) (ABIN458012)
抗原
See all Lactoferrin (LTF) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Human lactoferrin, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Human lactoferrin
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交叉反应 （详细）
- The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus Monkey milk has been observed.
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产品特性
- In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. As reagent for the direct detection of lactoferrin in human cells, tissues and body fluids in immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from human milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lactotransferrin (LTF) antibody
LTF 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) antibodyLTF 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB22673 unconjugated
anti-Lactotransferrin (LTF) (AA 25-352) antibodyLTF 适用: 山羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 219-248) antibodyLTF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB11921 unconjugated
anti-Lactotransferrin (LTF) (AA 500-710) antibodyLTF 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (Internal Region) antibodyVerified LTF 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 63-193) antibodyLTF 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 25-352) antibodyLTF 适用: Cow WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) antibodyLTF 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactotransferrin (LTF) (AA 28-351) antibodyLTF 适用: 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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- Lactoferrin (LTF) (Lactotransferrin (LTF))
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别名
- Lactoferrin
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UniProt
- P02788
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途径
- Transition Metal Ion Homeostasis
抗原
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