Albumin 抗体 (HRP)
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- 抗原 See all Albumin (ALB) 抗体
- Albumin (ALB)
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Albumin antibody is conjugated to HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins frequently share antigenic determinants. of this antiserum has not been tested in detail.
- 产品特性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human albumin
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate the antibody activity to any other component of the human serum proteins. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product ALB Primary Antibody
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anti-Albumin (ALB) (AA 19-609) antibody
ALB 适用: 人 ELISA, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal 1G12-1B3 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (C-Term) antibodyALB 适用: 人 WB, IHC, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人, 猴 ELISA, WB, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 1F5H7 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人 ELISA, WB, IHC, FACS 宿主: 小鼠 Monoclonal 3F6E5 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 ELISA, WB, IF, FACS, Coat 宿主: 小鼠 Monoclonal ALB-2356 unconjugated
anti-Albumin (ALB) (AA 20-255) antibodyALB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (AA 540-569), (C-Term) antibodyALB 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18676 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 猕猴 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in human serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Enzyme/IgG protein molar ratio (E/P) is approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
- 缓冲液
- Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml ster ile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- Albumin (ALB)
- 别名
- Albumin (ALB 产品)
- 别名
- PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, ALB antibody, CSA antibody, Alb-1 antibody, Alb1 antibody, Albza antibody, LOC100136344 antibody, alb-a antibody, alb-b antibody, albumin antibody, serum albumin 1 antibody, albumin S homeolog antibody, ALB antibody, Alb antibody, LOC100136344 antibody, alb.S antibody
- 背景
- Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal human serum and purified human albumin. One characteristic precipitin line is obtained against pooled normal human serum using different antigen/antibody concentration ratio’s. Precipitin lines against normal human serum and purified human albumin give a reaction of full identity
- 途径
- Lipid Metabolism
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