Albumin 抗体 (Biotin)
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- 抗原 See all Albumin (ALB) 抗体
- Albumin (ALB)
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Albumin antibody is conjugated to Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested in detail.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal goat antiserum to human albumin
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological con-ditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product ALB Primary Antibody
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anti-Albumin (ALB) (AA 19-609) antibody
ALB 适用: 人 ELISA, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal 1G12-1B3 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (C-Term) antibodyALB 适用: 人 WB, IHC, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人, 猴 ELISA, WB, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 1F5H7 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人 ELISA, WB, IHC, FACS 宿主: 小鼠 Monoclonal 3F6E5 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 ELISA, WB, IF, FACS, Coat 宿主: 小鼠 Monoclonal ALB-2356 unconjugated
anti-Albumin (ALB) (AA 20-255) antibodyALB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (AA 540-569), (C-Term) antibodyALB 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18676 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 猕猴 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- In immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, in non-isotopic assay methodology (e.g. ELISA) to measure albumin in human serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 500, in ELISA and comparable non-precipitating antibody-binding assays between 1:5,000 and 1:50,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.3. No foreign proteins added.
- 缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- Albumin (ALB)
- 别名
- Albumin (ALB 产品)
- 别名
- PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, ALB antibody, CSA antibody, Alb-1 antibody, Alb1 antibody, Albza antibody, LOC100136344 antibody, alb-a antibody, alb-b antibody, albumin antibody, serum albumin 1 antibody, albumin S homeolog antibody, ALB antibody, Alb antibody, LOC100136344 antibody, alb.S antibody
- 背景
- The defined antibody specificity is directed to albumin as tested against human sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin.
- 途径
- Lipid Metabolism
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