IgA Secretory Component (Free & Bound) 抗体
There are 2+ publications for this product available. The 山羊 多克隆 anti-IgA Secretory Component antibody is suitable to detect IgA Secretory Component in samples from 人. It has been validated for IEP 和 RID.
(2 references)
产品编号 ABIN457970
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for IgA Secretory Component (Free & Bound) 抗体 (ABIN457970)
抗原
IgA Secretory Component
适用
人
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
-
-
抗原表位
- Free & Bound
-
原理
- Goat anti Human secretory component (free and bound)
-
特异性
- Precipitating polyclonal Goat antiSerum to Human secretory component
-
交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
-
产品特性
- Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in human serum or other body fluids and to determine its concentration.
-
纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
-
免疫原
- Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
-
-
应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
-
缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
-
-
-
: "Secretory anti-citrullinated protein antibodies in serum associate with lung involvement in early rheumatoid arthritis." in: Rheumatology (Oxford, England), Vol. 59, Issue 4, pp. 852-859, (2020) (PubMed).
: "Circulating secretory IgA antibodies against cyclic citrullinated peptides in early rheumatoid arthritis associate with inflammatory activity and smoking." in: Arthritis research & therapy, Vol. 18, Issue 1, pp. 119, (2017) (PubMed).
-
-
- IgA Secretory Component
-
别名
- Secretory Immunoglobulin A (SIgA)
抗原
-
You are here:
