SERPINC1 抗体
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北京 101111
Quick Overview for SERPINC1 抗体 (ABIN457962)
抗原
See all SERPINC1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Human antithrombin III
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特异性
- Precipitating polyclonal Goat antiSerum to Human antithrombin III
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交叉反应 （详细）
- The antiSerum does not cross react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction has been found with Rhesus Monkey.
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产品特性
- The reactivity of the antiserum is restricted to antithrombin III. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified antithrombin III. No reaction is obtained with any other plasma protein including the main proteinase inhibitors known to have structural homology with antithrombin III. Protein complexes containing antithrombin III may also react. The anodic mobility enables quantitative determination of antithrombin III using the standard EID technique (Laurell). Immunoelectrophoresis and radial immunodiffusion are used to identify antithrombin III and enzyme inhibitor complexes. Double radial immunodiffusion (Mancini) is also used for quantitative assays. If applied in more sensitive test procedures, in Immunohistochemistry, or solid phase immunoassays, appropriate specificity controls should always be included. Plasma samples and all assay components must contain EDTA to stabilize the proteins.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- Antithrombin II is a single polypeptide chain glycoprotein (MW 61,000) with a high degree of homology with the alpha-1 antitrypsin. It is a circulating natural anticoagulant acting as a proteinase inhibitor. It is synthesized in the liver and the vascular endothelial cells. In electrophoresis of plasma it migrates in the alpha-1 region and is consumed during coagulation in vivo. Antithrombin III inhibits the action of factors IXa, Xa, XIIa, thombin and kallikrein by forming a stable complex. Heparin acts as a cofactor which greatly increases its inhibitory activities. Normal adult plasma contain an average of 0.15 mg antithrombin III per ml. This can vary from 75 to 128%. A partial deficiency in antithrombin III activity in the plasma (less than 50%) will result in an increased tendency to recurrent thrombo-enbolism. Several types of congenital deficiency have been reported. Most common type 1 deficiency patients have reduced antithrombin III antigen and activity levels. Heterozygotes have levels of 25 to 50% of normal values. For use as an immunogen antithrombin III is isolated and highly purified from pooled plasma. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Serpin Family C Member 1 (SERPINC1) antibody
SERPINC1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 33-465) antibodySERPINC1 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 150-426) antibodySERPINC1 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 364-393), (C-Term) antibodySERPINC1 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB18489 unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 33-465) antibodySERPINC1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 1-259) antibodySERPINC1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B12 unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 33-185) antibodySERPINC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family C Member 1 (SERPINC1) antibodySERPINC1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 37-180) antibodySERPINC1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Serpin Family C Member 1 (SERPINC1) (AA 141-211) antibodySERPINC1 适用: 人 WB, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Precipitation assays. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C.
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- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
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别名
- Antithrombin III
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UniProt
- P01008
抗原
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