Bence-Jones Protein lambda (Chain lambda), (Free & Bound) 抗体 (Biotin)
The 小鼠 单克隆 anti-Bence-Jones Protein lambda antibody is suitable to detect Bence-Jones Protein lambda in samples from 人. It has been validated for ELISA 和 WB.
产品编号 ABIN457853
发货至:
中国
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Our Local Distributor
中国
北京 101111
北京 101111
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Room 801-803
Quick Overview for Bence-Jones Protein lambda (Chain lambda), (Free & Bound) 抗体 (Biotin) (ABIN457853)
抗原
Bence-Jones Protein lambda
适用
人
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
ELISA, Western Blotting (WB)
克隆位点
NI 412 and NI 268
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抗原表位
- Chain lambda, Free & Bound
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特异性
- The antibody does not react with any other component of the human immuno-globulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
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产品特性
- Biotin-conjugated purified monoclonal mouse antibody to human Ig lambda chain, free and bound
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纯化方法
- Purified
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免疫原
- Highly purified Bence Jones lambda proteins isolated from pooled human urine.
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亚型
- IgG1
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应用备注
- To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:100 upwards, in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) is approximately 2.7. No foreign proteins added.
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缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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- Bence-Jones Protein lambda
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别名
- Bence Jones lambda
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背景
- The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and indirect immunoperoxidase staining.
抗原
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