IgA Secretory Component (Membrane-Bound), (Soluble) 抗体
适用: 人
ELISA, WB, IF, H
宿主: 小鼠
Monoclonal
NI 194-4 (A89-039)
unconjugated
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- 抗原
- IgA Secretory Component
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抗原表位
- Membrane-Bound, Soluble
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Haemagglutination (H)
- 特异性
- The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
- 产品特性
- Purified monoclonal mouse antibody to human secretory component, free and bound
- 纯化方法
- Purified
- 免疫原
- Highly purified secretory component isolated from human milk.
- 克隆位点
- NI 194-4 (A89-039)
- 亚型
- IgG1 kappa
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- 应用备注
- To identify the presence of free or bound secretory component in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG concentration is 0.4 mg/ml. No foreign proteins added.
- 缓冲液
- Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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- 抗原
- IgA Secretory Component
- 别名
- Secretory Immunoglobulin A (SIgA)
- 背景
- The reactivity of the antiserum is restricted to determinants on the secretory component as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
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