Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
产品特性
Purified monoclonal mouse antibody to human lactoferrin
纯化方法
Purified
免疫原
Highly purified lactoferrin isolated from human milk.
To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:50 upwards, in Western blotting from 1:250 upwards. These data should be interpreted as general recommendations only.
限制
仅限研究用
状态
Lyophilized
浓度
IgG concentration is 0.4 mg/ml. No foreign proteins added.
缓冲液
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.