Ovalbumin 抗体 (FITC)
Our Local Distributor
北京 101111
Quick Overview for Ovalbumin 抗体 (FITC) (ABIN457639)
抗原
See all Ovalbumin (OVA) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- Rabbit anti Chicken ovalbumin, conjugated with FITC
-
特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Chicken ovalbumin.
-
交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
-
产品特性
- A single precipitin line is obtained in immunoelectrophoresis against 1 % chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1 % chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum. In immunocytochemical and immunohistochemical staining of ovalbumin at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
-
纯化方法
- Purified IgG fraction from antiserum
-
免疫原
- Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
anti-Ovalbumin (OVA) (AA 1-350) antibody
OVA 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibody (HRP)OVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
anti-Ovalbumin (OVA) antibodyOVA 适用: Various Species WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: Avian WB, ELISA 宿主: 小鼠 Monoclonal 3G2E1D9-3D5F4A8-3G1E1D9 unconjugated
-
-
应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
-
缓冲液
- FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
-
-
- Ovalbumin (OVA)
-
别名
- Ovalbumin
抗原
-
