Ovalbumin 抗体 (FITC)
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- 抗原 See all Ovalbumin (OVA) 抗体
- Ovalbumin (OVA)
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ovalbumin antibody is conjugated to FITC
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应用范围
- Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
- 产品特性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to chicken ovalbumin
- 纯化方法
- The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immuno-electrophoresis and double radial immunodiffusion.
- 免疫原
- Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product OVA Primary Antibody
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anti-Ovalbumin (OVA) (AA 1-350) antibody
OVA 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibody (HRP)OVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
anti-Ovalbumin (OVA) antibodyOVA 适用: Various Species WB, IP, IHC, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA 宿主: 小鼠 Monoclonal 3G2E1D9 unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: Avian WB, ELISA 宿主: 小鼠 Monoclonal 3G2E1D9-3D5F4A8-3G1E1D9 unconjugated
anti-Ovalbumin (OVA) antibody (Biotin)OVA 适用: 小鸡 WB, ELISA, IP, DB 宿主: 兔 Polyclonal Biotin
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- In immunocytochemical and immunohistochemical staining of ovalbumin at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7. No foreign proteins added.
- 缓冲液
- FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by ad ding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- Ovalbumin (OVA)
- 别名
- Ovalbumin (OVA 产品)
- 背景
- A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum
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