Albumin 抗体 (HRP)
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北京 101111
Quick Overview for Albumin 抗体 (HRP) (ABIN457585)
抗原
See all Albumin (ALB) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Bovine albumin, conjugated with Horseradish peroxidase
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特异性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine albumin.
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交叉反应
- Cat, 犬, 山羊, Hamster, 马, 人, 猴, Pig, 绵羊
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无交叉反应
- 小鸡, 鸭, 兔
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in double radial immunodiffusion with the following results: Cat + Chicken - Dog + Duck - Goat ++ Guinea Pig ± Hamster + Horse + Human + Monkey + Mouse ± pigeon - Rabbit - Rat ± Sheep ++ Swine + A negative result in this test does not exclude some cross-reaction in more sensitive techniques.
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产品特性
- Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal bovine serum and purified bovine albumin. One characteristic precipitin line is obtained against pooled normal bovine serum using different antigen/antibody concentration ratio's. Precipitin lines against normal bovine serum and purified bovine albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in bovine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Purified albumin isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Albumin (ALB) (AA 19-609) antibody
ALB 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 1G12-1B3 unconjugated
anti-Albumin (ALB) (AA 540-569), (C-Term) antibodyALB 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18676 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人, 猴 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 1F5H7 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3F6E5 unconjugated
anti-Albumin (ALB) (C-Term) antibodyALB 适用: 人 WB, IHC, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal ALB-2356 unconjugated
anti-Albumin (ALB) (AA 20-255) antibodyALB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal ALB-2355 unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- Albumin (ALB)
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别名
- Albumin
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途径
- Lipid Metabolism
抗原
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