CD3 抗体
(9 references)-
- 抗原 See all CD3 抗体
- CD3
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- 特异性
- The mouse monoclonal antibody OKT3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 内毒素水平
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
- 免疫原
- human T cells
- 克隆位点
- OKT3
- 亚型
- IgG2a
- Top Product
- Discover our top product CD3 Primary Antibody
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (APC)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (PE)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE
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- 应用备注
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Functional application: counteracting both generation and function of effector T cells.
Flow cytometry: Recommended dilution: 1 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4
- 储存液
- Azide free
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients." in: Respiratory research, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).
: "Matrine induces cell anergy in human Jurkat T cells through modulation of mitogen-activated protein kinases and nuclear factor of activated T-cells signaling with concomitant up-regulation of ..." in: Biological & pharmaceutical bulletin, Vol. 33, Issue 1, pp. 40-6, (2010) (PubMed).
: "T cell activation requires mitochondrial translocation to the immunological synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14418-23, (2007) (PubMed).
: "Human immunodeficiency virus reactivation by phorbol esters or T-cell receptor ligation requires both PKCalpha and PKCtheta." in: Journal of virology, Vol. 79, Issue 15, pp. 9821-30, (2005) (PubMed).
: "Antigen-independent acquisition of MHC class II molecules by human T lymphocytes." in: International immunology, Vol. 16, Issue 10, pp. 1523-33, (2004) (PubMed).
: "Transplantation of highly purified CD34+ progenitor cells from unrelated donors in pediatric leukemia." in: Blood, Vol. 101, Issue 4, pp. 1630-6, (2003) (PubMed).
: "A large-scale method for T cell depletion: towards graft engineering of mobilized peripheral blood stem cells." in: Bone marrow transplantation, Vol. 30, Issue 2, pp. 69-74, (2002) (PubMed).
: "OKT-3-based reconditioning regimen for early graft failure in HLA-non-identical stem cell transplants." in: British journal of haematology, Vol. 111, Issue 2, pp. 668-73, (2001) (PubMed).
: "Ten years of Orthoclone OKT3 (muromonab-CD3): a review." in: Journal of transplant coordination : official publication of the North American Transplant Coordinators Organization (NATCO), Vol. 6, Issue 3, pp. 109-19; quiz 120-1, (1997) (PubMed).
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: "Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients." in: Respiratory research, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).
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- 抗原
- CD3
- 别名
- CD3 (CD3 产品)
- 别名
- CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody
- 背景
- CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE
- 基因ID
- 916
- UniProt
- P07766
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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