MHC Class II 抗体 (FITC)
(8 references)Our Local Distributor
北京 101111
Quick Overview for MHC Class II 抗体 (FITC) (ABIN457349)
抗原
See all MHC Class II (MHC2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-Ms MHC Class II FITC
-
特异性
- The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
-
交叉反应 （详细）
- Mouse
-
纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
-
免疫原
- Activated C57BL/6 mouse spleen cells
-
亚型
- IgG2b
-
-
anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ICC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠 FACS, WB, IP, IHC (fro), Func, IHC (p) 宿主: 大鼠 Monoclonal M5-114 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)MHC2 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (SPRD)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 SPRD
-
-
应用备注
- Flow cytometry: Recommended dilution: 2-3 μg/mL.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
-
Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
-
-
-
: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).
: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).
: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).
: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." in: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).
: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." in: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).
: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." in: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).
: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).
: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
-
: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).
-
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
-
别名
- MHC Class II
-
背景
- MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
抗原
-
