TLN1 抗体 (Internal Region)
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- 抗原 See all TLN1 抗体
- TLN1 (Talin 1 (TLN1))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠, Cow, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This TLN1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Recognizes Talin-1 (TLN1). This antibody is not expected to cross-react with the highly similar protein Talin 2.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human. - 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence C-QEALHTQMLTAVQE, from the internal region of Human Talin 1 (NP_006280.3.)
- Top Product
- Discover our top product TLN1 Primary Antibody
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anti-Talin 1 (TLN1) (AA 1052-1149) antibody
TLN1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1A11 unconjugated
anti-Talin 1 (TLN1) (AA 975-1181) antibodyTLN1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Talin 1 (TLN1) (AA 1-220) antibodyTLN1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Talin 1 (TLN1) (AA 1601-1750) antibody (FITC)TLN1 适用: 小鼠, 大鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-Talin 1 (TLN1) (AA 1324-1424) antibodyTLN1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G4 unconjugated
anti-Talin 1 (TLN1) (AA 1324-1424) antibodyTLN1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C8 unconjugated
anti-Talin 1 (TLN1) (AA 1052-1150) antibodyTLN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1A11 unconjugated
anti-Talin 1 (TLN1) (AA 1601-1750) antibodyTLN1 适用: 小鼠, 大鼠 ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Talin 1 (TLN1) (AA 2293-2536) antibodyTLN1 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Talin 1 (TLN1) (AA 92-399) antibodyTLN1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Talin 1antibody Detection limit dilution: 1/8000. Western blot: 1-3 μg/mL. A >250 kDa band observed in Human Peripheral Mononucleocytelysates (calculated MW of 270 kDa according to NP_006280.3). An additional band of unknown identity was also consistently observed at 110 kDa. Thisband was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- TLN1 (Talin 1 (TLN1))
- 别名
- Talin-1 (TLN1) (TLN1 产品)
- 别名
- ILWEQ antibody, TLN antibody, Tln antibody, sb:cb854 antibody, wu:fb05c02 antibody, TLN1 antibody, talin-1 antibody, tln antibody, talin 1 antibody, talin 1 L homeolog antibody, Talin 1 antibody, talin-1 antibody, TLN1 antibody, Tln1 antibody, tln1 antibody, tln1.L antibody, Bm1_56490 antibody, LOC100380665 antibody
- 背景
- Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.Synonyms: KIAA1027, TLN
- 基因ID
- 7094
- NCBI登录号
- NP_006280
- UniProt
- Q9Y490
- 途径
- Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location
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