SOD3 抗体
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北京 101111
Quick Overview for SOD3 抗体 (ABIN453955)
抗原
See all SOD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody detects EC-SOD at ~35 kDa. Does not cross react with other SODs.
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交叉反应 （详细）
- Species reactivity (tested):Human, Rat, Mouse.
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产品特性
- Synonyms: SOD-3, EC-SOD, Extracellular superoxide dismutase [Cu-Zn]
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纯化方法
- Protein G Chromatography
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免疫原
- Human EC-SOD purified from aortas
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亚型
- IgG1
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anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 19-240) antibody
SOD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) antibodySOD3 适用: 人 WB, IHC, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 4GG11G6 unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 16-244) antibodySOD3 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 19-240) antibodySOD3 适用: 人 WB 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 19-160) antibodySOD3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 7-36), (N-Term) antibodySOD3 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19866 unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 7-36) antibodySOD3 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) (AA 25-251) antibodySOD3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 3, Extracellular (SOD3) antibody (Atto 488)SOD3 适用: 人 WB, IHC, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 4GG11G6 Atto 488
anti-Superoxide Dismutase 3, Extracellular (SOD3) antibody (Atto 390)SOD3 适用: 人 WB, IHC, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 4GG11G6 Atto 390
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应用备注
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Western blot: 1/1000.1 μg/mL was sufficient for detection of EC-SOD in 20 μg of Human cartillage lysate bycolorimetric Immunoblot using Goat anti-Mouse IgG (HRP) as the secondary antibodyELISA (7). Immunocytochemistry: 1/100. Immunohistochemistry on Frozen Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 50 % Glycerol
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))
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别名
- Superoxide Dismutase 3 / SOD3
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背景
- Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: EC-SOD, Extracellular superoxide dismutase [Cu-Zn], SOD-3
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基因ID
- 6649
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UniProt
- P08294
抗原
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