IER5 抗体 (Internal Region)
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Quick Overview for IER5 抗体 (Internal Region) (ABIN452683)
抗原
See all IER5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody recognizes IER5.
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-QPPSGGEDDDAEE, from the internal region of the protein sequence according to NP_057629.1.
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应用备注
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Peptide ELISA: Antibody Detection Limit Dilution: 1/2000. Western Blot: 1-3 μg/mL. Customer's experiments gave a band at approx 45 kDa in lysatesof transfected H1299 overexpressing IER5 but not when transfected with empty vector(calculated MW of 33.5 kDa according to NP_057629.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- IER5 (Immediate Early Response 5 (IER5))
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别名
- IER5
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背景
- IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.Synonyms: Immediate early response gene 5 protein, PP4583, SBBI48
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基因ID
- 51278
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NCBI登录号
- NP_057629
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UniProt
- Q5VY09
抗原
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