SDHAF1 抗体 (C-Term)
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Quick Overview for SDHAF1 抗体 (C-Term) (ABIN452676)
抗原
See all SDHAF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- c-HDSTGAPETR PDGR
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特异性
- This antibody detects Human SDHAF1 at C-term.
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Canine (Dog).
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide with sequence from the C-Terminus of the protein sequence according to NP_001036096.1. Genename: RNF31
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应用备注
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Peptide ELISA: Detection limit dilution 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 19 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 12.9 kDa according to NP_001036096.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- SDHAF1 (Succinate Dehydrogenase Complex Assembly Factor 1 (SDHAF1))
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别名
- SDHAF1
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背景
- The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). SDHAF1 Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II (Probable)Synonyms: LYR motif-containing protein 8, SDH assembly factor 1, Succinate dehydrogenase assembly factor 1 mitochondrial
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基因ID
- 644096
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NCBI登录号
- NP_001036096
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UniProt
- A6NFY7
抗原
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