Aflatoxin 抗体
AFT
适用: Aspergillus
LF, EIA
宿主: 大鼠
Monoclonal
2-00E-004
unconjugated
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- 抗原 See all Aflatoxin (AFT) products
- Aflatoxin (AFT)
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适用
- Aspergillus
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Aflatoxin antibody is un-conjugated
- 应用范围
- Lateral Flow (LF), Enzyme Immunoassay (EIA)
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Winstar rats were immunized with Aflatoxin M1-BSA conjugate.
- 克隆位点
- 2-00E-004
- 亚型
- IgG2b
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- 应用备注
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ELISA: 100 ng of AFM1 can be detected as competitive conjugate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with double distillated water to adjust the final concentration to 1.0 mg/mL.
- 缓冲液
- 0.01 M PBS, pH 7.2 without preservatives
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody in aliquots at -20 °C after reconstitution. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Aflatoxin (AFT)
- 别名
- Aflatoxin (AFT 产品)
- 物质类
- Chemical
- 背景
- The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA and protein. Cytochrome P450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1?Molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals.
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