HSP70 抗体
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北京 101111
Quick Overview for HSP70 抗体 (ABIN452399)
抗原
See all HSP70 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody detects several members of the heat shock protein 70 kDa gene family including Hsp70, Hsc70, Grp78, and following heat shock, Hsp72. Immunfluorescence staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse, Rat, Amphibian, Bird, Fish, Rabbit, Saccharomyces cerevisiae, Fruit Fly
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产品特性
- Synonyms: HSP70.1, HSP70-1/HSP70-2, HSPA1A, HSPA1B, HSPA1, Heat shock 70 kDa protein 1A/1B
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纯化方法
- Protein G Affinity Chromatography
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免疫原
- Human recombinant Hsp70 over expressed in E. coli
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亚型
- IgG1
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anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, ICC, FACS, EM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated
anti-Heat Shock Protein 70 (HSP70) antibodyHSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 70 (HSP70) antibody (PE)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PE
anti-Heat Shock Protein 70 (HSP70) antibody (APC)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal APC
anti-Heat Shock Protein 70 (HSP70) antibody (FITC)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal FITC
anti-Heat Shock Protein 70 (HSP70) antibody (HRP)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal HRP
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 390)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 390
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 594)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 594
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 488
anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
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Immunocytochemistry. Immunohistochemistry on frozen sections. Immunflourescence. Immunoprecipitation. Western blot: 1 - 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS pH 7.2, 50 % Glycerol, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Shelf life: one year from despatch. -
有效期
- 12 months
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- HSP70 (Heat Shock Protein 70 (HSP70))
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别名
- Heat Shock Protein 70 / HSP70
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背景
- Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.Synonyms: HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B
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基因ID
- 3303
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UniProt
- P08107
抗原
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