Recombinant Blue Fluorescent Protein 抗体 (Atto 488)
This anti-Blue Fluorescent Protein antibody is a Alpaca 单克隆 antibody detecting Blue Fluorescent Protein in FACS, ICC 和 IF. Suitable for Entacmaea quadricolor.
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Quick Overview for Recombinant Blue Fluorescent Protein 抗体 (Atto 488) (ABIN4368359)
抗原
Blue Fluorescent Protein (BFP)
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
适用
Entacmaea quadricolor
宿主
Alpaca
克隆类型
单克隆
标记
This Blue Fluorescent Protein antibody is conjugated to Atto 488
应用范围
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
克隆位点
1H7
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Expression System
- E.coli
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原理
- Camelid sdAb anti-TagFP conjugated with Atto488, Clone 1H7
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特异性
- Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor
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交叉反应 （详细）
- Does not cross-react with common GFP- or dsRed derivatives.
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产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
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纯化方法
- Produced in: E.coli
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免疫原
- BFP
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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应用备注
- Recommended dilution 1:500
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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浓度
- 5 μM
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缓冲液
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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注意事项
- Protect from light!
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储存条件
- -20 °C,-80 °C
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储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
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有效期
- 12 months
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- Blue Fluorescent Protein (BFP)
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别名
- TagBFP
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分子量
- 26 kDa
抗原
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