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RHOT1 抗体 (N-Term)

Name: RHOT1 抗体 (N-Term)
SKU: ABIN405704
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-RHOT1 antibody is a 兔多克隆 antibody detecting RHOT1 in WB. Suitable for 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔和斑马鱼. Independently validated for use in Western Blotting.

Western Blotting validation image for anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibody (ABIN405704) (RHOT1 抗体 (N-Term))

WB Suggested Anti-RHOT1 Antibody Titration: 0. (RHOT1 抗体 (N-Term))

2 images

(1 validation)

产品编号 ABIN405704

发货至: 中国

Quick Overview for RHOT1 抗体 (N-Term) (ABIN405704)

抗原

See all RHOT1 抗体

RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

适用

人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼

宿主

兔

克隆类型

多克隆

标记

This RHOT1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product RHOT1 Primary Antibody

抗原表位
8

6

3

2

2

1

1

1

1

1
N-Term

序列

EMKPACIKAL TRIFKISDQD NDGTLNDAEL NFFQRICFNT PLAPQALEDV

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

产品特性

This is a rabbit polyclonal antibody against RHOT1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human RHOT1
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibody
RHOT1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibody
RHOT1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 483-580) antibody
RHOT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H4 unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibody
RHOT1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, Bat, 小鸡, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibody
RHOT1 适用: 人, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 483-580) antibody
RHOT1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A12 unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 483-580) antibody
RHOT1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 1-659) antibody
RHOT1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 396-445) antibody
RHOT1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 180-229) antibody
RHOT1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 659 AA

限制

仅限研究用
生效 #100008 (Western Blotting)

by

Shaw Lab, Department of Biochemistry, University of Utah

No.

#100008

日期

2015.09.12

抗原

RHOT1

Lot Number

Method validated

Western Blotting

Positive Control

Purified recombinant Miro1

Isolated mitochondria from immortalized Miro1 KO MEFs expressing Myc-Miro1

Negative Control

Purified recombinant Miro2

Isolated mitochondria from immortalized Miro1 WT MEFs

Isolated mitochondria from immortalized Miro1 KO MEFs expressing Myc-Miro2

Notes

Passed. ABIN405704 specifically recognizes Miro1 without cross-reaction with Miro2.

Validation Images
Full Methods
Primary Antibody

ABIN405704

Secondary Antibody

Goat-anti-rabbit IgG HRP conjugated (Sigma, A0545-1M22)

Full Protocol

Transfect immortalized Miro1-/- MEFs with Myc-Miro1-V1 or Myc-Miro2-V1 as described in PMID 25136135.
Isolate mitochondria from immortalized Miro1+/+, Miro1-/-, Miro1-/- + Myc-Miro1 and Miro1-/- + Myc-Miro2 MEFs.
Determine protein concentration and snap freeze mitochondria in liquid nitrogen.
Thaw 30 µg mitochondria per lane on ice.
Thaw 100 ng recombinant Miro1 and Miro2 per lane on ice.
Add 2x Laemmli buffer and 2% BME to samples and denature for 5 min at 95 °C.
Separte 30 µg/lane mitochondria and 100 ng/lane recombinant protein on a 12.5% SDS-PAGE for 3 h at 40mA/180V.
Semi-dry transfer to PVDF membrane for 2 h at 250 mA/15 V.
Block the membrane with TBST + 5% non-fat dry milk for 1 h at RT.
Incubate with primary
rabbit anti-RHOT1 antibody (antibodies-online, ABIN405704) diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
rabbit anti-Myc1 antibody diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
rabbit anti-Cyt c antibody diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
Wash membrane 3x for 5 min with TBST buffer.
Incubate with secondar goat anti-rabbit IgG HRP antibody (Sigma, A0545-1M2) diluted 1:2000 in TBST + 5% non-fat dry milk for 1 h at RT.
Wash membrane 3x for 10 min with TBST buffer.
Reveal protein bands on a Bio-Rad imaging system using homemade ECL prepared according to PMID 17141265.
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Experimental Notes
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

别名

RHOT1

背景

RHOT1 is mitochondrial GTPase involved in mitochondrial trafficking. It is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Alias Symbols: ARHT1, FLJ11040, FLJ12633, MIRO-1, MIRO1
Protein Interaction Partner: PCSK9, ERLIN2, PARK2, ILK, UBC, CFTR, PINK1, UBD, IRAK1, AMFR,
Protein Size: 659

分子量

75 kDa

基因ID

55288

NCBI登录号

NM_001033566, NP_001028738

UniProt

Q8IXI2