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Myc Tag 抗体 (HRP)

This anti-Myc Tag antibody is a 兔 多克隆 antibody detecting Myc Tag in WB, IHC (fro) 和 EIA. Suitable for .
产品编号 ABIN401754
发货至: 中国

Quick Overview for Myc Tag 抗体 (HRP) (ABIN401754)

抗原

Myc Tag

适用

请咨询

宿主

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克隆类型

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多克隆

标记

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This Myc Tag antibody is conjugated to HRP

应用范围

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Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
  • 序列

    EQKLISEEDL

    特异性

    This antibody is directed against human c-myc.

    产品特性

    Absorption / Emission: 770 nm / 794 nm
    Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG

    纯化方法

    Affinity chromatography

    免疫原

    Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
  • 应用备注

    ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.

    限制

    仅限研究用
  • 溶解方式

    Restore with 0.1 mL of deionized water (or equivalent).

    浓度

    1.0 mg/mL (by UV absorbance at 280 nm)

    缓冲液

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative

    储存液

    Gentamicin sulfate

    注意事项

    Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Myc Tag

    别名

    C-Myc Epitope Tag

    物质类

    Tag

    背景

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag

    基因ID

    4609

    NCBI登录号

    NP_002458

    UniProt

    P01106
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