Cofilin 抗体 (pTyr140)
Quick Overview for Cofilin 抗体 (pTyr140) (ABIN401586)
抗原
See all Cofilin (CFL1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr140
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特异性
- Antibody AP09483PU detects endogenous levels of Cofilin-1 only when phosphorylated at Tyrosine 139.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human cofilin1 around the phosphorylation site of Tyrosine 139 (N-C-Yp-E-E).
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anti-Cofilin 1 (CFL1) (AA 1-166) antibody
CFL1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-Cofilin 1 (CFL1) antibodyKO Validated CFL1 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (AA 3-161) antibodyCFL1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (pSer3) antibodyCFL1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (AA 1-100) antibodyCFL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (AA 2-166) antibodyCFL1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (AA 70-98) antibodyCFL1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB23947 unconjugated
anti-Cofilin 1 (CFL1) (AA 4-144) antibodyCFL1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (pSer3) antibodyCFL1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cofilin 1 (CFL1) (pSer3) antibodyCFL1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C.
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- Cofilin (CFL1) (Cofilin 1 (CFL1))
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别名
- Cofilin-1
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背景
- Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH -dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18
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基因ID
- 1072
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NCBI登录号
- NP_005498
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UniProt
- P23528
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途径
- Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
抗原
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