GAP43 抗体 (pSer41)
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Quick Overview for GAP43 抗体 (pSer41) (ABIN401572)
抗原
See all GAP43 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer41
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特异性
- Antibody AP09476PU detects endogenous levels of GAP43 only when phosphorylated at Ser41.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human GAP43 around the phosphorylation site of Ser41 (Q-A-Sp-F-R).
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应用备注
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Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C.
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- GAP43 (Growth Associated Protein 43 (GAP43))
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别名
- GAP43
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背景
- GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding.Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46
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基因ID
- 2596
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NCBI登录号
- NP_002036
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UniProt
- P17677
抗原
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