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EIF4B 抗体 (AA 420-424)

The 兔 多克隆 anti-EIF4B antibody (ABIN401568) specifically detects EIF4B in WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN401568
发货至: 中国
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Quick Overview for EIF4B 抗体 (AA 420-424) (ABIN401568)

抗原

See all EIF4B 抗体
EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

适用

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  • 1

宿主

  • 107
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克隆类型

  • 86
  • 34
多克隆

标记

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This EIF4B antibody is un-conjugated

应用范围

  • 104
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 30
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    AA 420-424

    特异性

    This antibody detects endogenous levels of total eIF4B protein.

    纯化方法

    Immunoaffinity Chromatography using epitope-specific peptide.

    免疫原

    Peptide sequence around amino acids 420~424 (T-G-S-E-S) derived from Human eIF4B.
  • 应用备注

    Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody (in aliquots) at-20 °C.
  • 抗原

    EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

    别名

    EIF4B

    背景

    EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B

    基因ID

    1975

    NCBI登录号

    NP_001408

    UniProt

    P23588
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