EIF4B 抗体 (AA 420-424)
Our Local Distributor
北京 101111
Quick Overview for EIF4B 抗体 (AA 420-424) (ABIN401568)
抗原
See all EIF4B 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 420-424
-
特异性
- This antibody detects endogenous levels of total eIF4B protein.
-
纯化方法
- Immunoaffinity Chromatography using epitope-specific peptide.
-
免疫原
- Peptide sequence around amino acids 420~424 (T-G-S-E-S) derived from Human eIF4B.
-
-
-
-
应用备注
-
Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Store the antibody (in aliquots) at-20 °C.
-
-
- EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))
-
别名
- EIF4B
-
背景
- EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B
-
基因ID
- 1975
-
NCBI登录号
- NP_001408
-
UniProt
- P23588
抗原
-