MARCKS 抗体 (pSer170)
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北京 101111
Quick Overview for MARCKS 抗体 (pSer170) (ABIN401558)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer170
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特异性
- This antibody detects endogenous levels of MARCKS only when phosphorylated at Threonine 170.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 170 (G-F-Sp-F-K).
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-65) antibody
MARCKS 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 1-100) antibodyMARCKS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibodyMARCKS 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer162) antibodyMARCKS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer158) antibodyMARCKS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Internal Region) antibodyMARCKS 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibodyMARCKS 适用: 人, 小鼠 WB, ELISA, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer152), (Ser156) antibodyMARCKS 适用: 人, 小鼠, 大鼠, Cow, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 31-120) antibodyMARCKS 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159) antibodyMARCKS 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1/500approx. 1/1000. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
- MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
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基因ID
- 4082
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NCBI登录号
- NP_002347
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UniProt
- P29966
抗原
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