GATA3 抗体 (N-Term)
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Quick Overview for GATA3 抗体 (N-Term) (ABIN401530)
抗原
See all GATA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- EVTADQPRWV SHH-C
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特异性
- This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
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anti-GATA Binding Protein 3 (GATA3) antibody
GATA3 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1A10A11 unconjugated
anti-GATA Binding Protein 3 (GATA3) (N-Term) antibodyGATA3 适用: 人, 小鼠, 大鼠, Cow, Pig, 斑马鱼, 犬, 兔, 马, 豚鼠, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 1-260) antibodyGATA3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (pSer308) antibodyGATA3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 103-200) antibodyGATA3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C1 unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 103-200) antibodyGATA3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A4 unconjugated
anti-GATA Binding Protein 3 (GATA3) (C-Term) antibodyGATA3 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 豚鼠, 绵羊 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 1-444) antibodyGATA3 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 1259CT707-136-102 unconjugated
anti-GATA Binding Protein 3 (GATA3) antibodyGATA3 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (full length) antibodyGATA3 适用: 人 IHC 宿主: 小鼠 Monoclonal 450M unconjugated
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应用备注
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Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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: "Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
: "Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1221-8, (2016) (PubMed).
: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).
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: "Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
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- GATA3 (GATA Binding Protein 3 (GATA3))
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别名
- GATA3
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背景
- The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
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基因ID
- 2625
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NCBI登录号
- NP_001002295
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UniProt
- P23771
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途径
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
抗原
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