SNX26 抗体 (C-Term)
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北京 101111
Quick Overview for SNX26 抗体 (C-Term) (ABIN401527)
抗原
See all SNX26 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- SWSLHSEGQT RSYC
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特异性
- This antibody detects SNX26 at C-term.
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交叉反应 （详细）
- Species reactivity (expected):MouseSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_443180.2. Genename: SNX26
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anti-Sorting Nexin 26 (SNX26) (N-Term) antibody
Verified SNX26 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Sorting Nexin 26 (SNX26) (C-Term) antibodyVerified SNX26 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Sorting Nexin 26 (SNX26) (C-Term) antibodySNX26 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 75 kDa band in Human Bone Marrow,Kidney, and Pancreas lysates after 1 μg/mL antibody staining. ABIN401527 may detect theisoform A1A5D2 (UniProtKB) with a molecular weight of 81.5 kDa which is similar in aminoacid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently wecannot find a precise explanation in the literature for the band we observe given thecalculated size of 125 kDa according to NP_443180.2. The 75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry on paraffin sections: 2.5 μg/mL. In Human Brain shows cytoplasmstaining of Purkinje cells in Cerebellum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- SNX26 (Sorting Nexin 26 (SNX26))
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别名
- Sorting Nexin-26 (SNX26)
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背景
- SNX26 may be involved in several stages of intracellular trafficking. It could play an important role in the regulation of glucose transport by insulin. It may act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.Synonyms: ARHGAP33, Rho GTPase-activating protein 33, TCGAP
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基因ID
- 115703
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NCBI登录号
- NP_443180
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UniProt
- O14559
抗原
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