CA1 抗体 (Internal Region)
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Quick Overview for CA1 抗体 (Internal Region) (ABIN401513)
抗原
See all CA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- c-QAIKTKGKRA P
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特异性
- This antibody detects internal Carbonic anhydrase 1.
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交叉反应 (详细)
- Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region of the protein sequence according to NP_001729.1 . Genename: CA1
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应用备注
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Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 29 kDa band observed in Human Liver lysates(calculated MW of 28.9 kDa according to NP_001729.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- CA1 (Carbonic Anhydrase I (CA1))
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别名
- Carbonic Anhydrase 1
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背景
- Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.Synonyms: CA-I, CA1, CAB, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I
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基因ID
- 759
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NCBI登录号
- NP_001729
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UniProt
- P00915
抗原
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