FITC 抗体 (Biotin)
Our Local Distributor
北京 101111
Quick Overview for FITC 抗体 (Biotin) (ABIN401490)
抗原
宿主
克隆类型
标记
应用范围
-
-
产品特性
- Molar Ratio: 10-20 BAC molecules per Goat F(ab')2 Molecule
-
纯化方法
- Immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
-
免疫原
- Fluorescein conjugated to Goat IgG
-
亚型
- IgG
-
-
-
-
应用备注
-
ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 2,000 - 1: 10,000. Immunohistochemistry: 1: 1,000 - 1: 5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
-
浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
-
缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
-
-
- FITC (Fluorescein Isothiocyanate (FITC))
-
别名
- FITC
-
物质类
- Chemical
-
背景
- Synonyms: Fluorescein Isothiocyanate
抗原
-