FITC 抗体 (Biotin)
Quick Overview for FITC 抗体 (Biotin) (ABIN401490)
抗原
适用
宿主
克隆类型
标记
应用范围
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产品特性
- Molar Ratio: 10-20 BAC molecules per Goat F(ab')2 Molecule
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纯化方法
- Immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
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免疫原
- Fluorescein conjugated to Goat IgG
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亚型
- IgG
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anti-Fluorescein Isothiocyanate (FITC) antibody
FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC WB 宿主: 兔 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibody (HRP)FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal HRP
anti-Fluorescein Isothiocyanate (FITC) antibody (Alkaline Phosphatase (AP))FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal Alkaline Phosphatase (AP)
anti-Fluorescein Isothiocyanate (FITC) antibody (Biotin)FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal Biotin
anti-Fluorescein Isothiocyanate (FITC) antibody (PE)FITC ELISA, FACS 宿主: 绵羊 Polyclonal PE
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应用备注
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ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 2,000 - 1: 10,000. Immunohistochemistry: 1: 1,000 - 1: 5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
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- FITC (Fluorescein Isothiocyanate (FITC))
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别名
- FITC
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物质类
- Chemical
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背景
- Synonyms: Fluorescein Isothiocyanate
抗原
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