LacI 抗体
This 兔 多克隆 antibody specifically detects LacI in WB 和 EIA. It exhibits reactivity toward E. coli 和 Shigella sonnei.
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产品编号 ABIN401451
发货至:
中国
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Quick Overview for LacI 抗体 (ABIN401451)
抗原
LacI
适用
E. coli, Shigella sonnei
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA)
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特异性
- This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100 % homology with the immunizing sequence.
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纯化方法
- Affinity Chromatography
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免疫原
- Full-length recombinant Lac I protein
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亚型
- IgG
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anti-LacI antibody
适用: E. coli, Shigella sonnei WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-LacI antibody适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/90,000-1/100,000. Western Blot: 1/500-1/2,000. Expect a band approximately approx. 38 kDa in size corresponding to Lac I by Western blotting inthe appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- LacI
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背景
- The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.Synonyms: JW0336, Lactose operon repressor, b0345
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基因ID
- 945007
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NCBI登录号
- AP_000997
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UniProt
- P03023
抗原
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