RNF25 抗体 (C-Term)
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Quick Overview for RNF25 抗体 (C-Term) (ABIN401373)
抗原
See all RNF25 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody is directed against RNF25 protein.
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纯化方法
- Affinity chromatography
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免疫原
- Synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein
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亚型
- IgG
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anti-Ring Finger Protein 25 (RNF25) (AA 1-459) antibody
RNF25 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 352-459) antibodyRNF25 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D10 unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 1-459) antibodyRNF25 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (C-Term) antibodyRNF25 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (Middle Region) antibodyRNF25 适用: 人, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 377-439) antibodyRNF25 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 201-250) antibodyRNF25 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Hamster, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (Middle Region) antibodyRNF25 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Cow, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 27-241) antibodyRNF25 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (C-Term) antibodyRNF25 适用: 人, 小鼠 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- RNF25 (Ring Finger Protein 25 (RNF25))
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别名
- RNF25
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背景
- RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kappaB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25
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基因ID
- 64320
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NCBI登录号
- NP_071898
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UniProt
- Q96BH1
抗原
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