TUBA1B 抗体 (AA 427-441)
Quick Overview for TUBA1B 抗体 (AA 427-441) (ABIN401310)
抗原
See all TUBA1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 427-441
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特异性
- This antibody is directed against alpha Tubulin protein. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent Loading Control.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin
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亚型
- IgG
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anti-Tubulin, alpha 1B (TUBA1B) antibody
TUBA1B 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF, FM, MA 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 425-451) antibodyTUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 29-310) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 437-441) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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ELISA: 1/5,000-1/25,000. Immunofluorescence: 1/500-1/2,000Western Blot: 1/500-1/3,000. Expect a band at approx. 50 kDa in size corresponding to alpha tubulin by Western blotting inmost cell lysates or extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.08 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- TUBA1B (Tubulin, alpha 1B (TUBA1B))
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别名
- alpha Tubulin / TUBA1B
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背景
- Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain
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基因ID
- 10376
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NCBI登录号
- NP_006073
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UniProt
- P68363
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途径
- Microtubule Dynamics, M Phase
抗原
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