SLIT1 抗体 (AA 487-504)
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Quick Overview for SLIT1 抗体 (AA 487-504) (ABIN401297)
抗原
See all SLIT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 487-504
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特异性
- This antibody is directed against SLIT-1 protein.
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交叉反应 (详细)
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Species reactivity (expected):Rat, Human, Chicken Frog.
Species reactivity (tested):Mouse. -
纯化方法
- Affinity chromatography
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免疫原
- Synthetic peptide corresponding aa 487-504 of mouse SLIT-1 protein
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亚型
- IgG
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应用备注
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ELISA: 1/10,000 - 1/50,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.2 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- SLIT1 (Slit Homolog 1 (SLIT1))
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别名
- SLIT1
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背景
- SLIT-1 (also known as KIAA0813, MEGF4, multiple epidermal growth factor-like domains 4 and Slit homolog 1 protein) is a Slit protein. This protein is a ligand for the Roundabout (Robo) receptors and acts as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. In situ hybridization studies indicated that the rat SLIT-1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. This data suggests that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Alternative splicing isoforms have been identified for Slit1 protein.Synonyms: KIAA0813, MEGF4, Multiple epidermal growth factor-like domains protein 4, SLIL1, SLIT-1, Slit homolog 1 protein
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基因ID
- 20562
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NCBI登录号
- NP_056563
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UniProt
- Q80TR4
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途径
- Regulation of Cell Size
抗原
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