SLIT2 抗体 (AA 484-500)
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Quick Overview for SLIT2 抗体 (AA 484-500) (ABIN401286)
抗原
See all SLIT2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 484-500
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特异性
- This antibody is directed against SLIT-2 protein.
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交叉反应 (详细)
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Species reactivity (expected):Chicken, Mouse, Rat, Bovine, Xenopus laevis.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography
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免疫原
- Synthetic peptide corresponding aa 484-500 of Human SLIT-2 protein.
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亚型
- IgG
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应用备注
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ELISA: 1/1,500 - 1/6,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted 2-8 °C fo one month or (in aliquots) at-20 °C for longer.
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- SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
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别名
- SLIT2
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背景
- SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors. SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play role in guiding commissural axons once they reach the floor plate by modulating the response to netrin. In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growthstimulatory effect and silencing requires the formation of a ROBO1-DCC complex. In vitro, only commissural axons that crossed the midline responded to SLIT-2. In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord. Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined. Multiple isoforms have been reported for this product.Synonyms: SLIL3, SLIT-2, Slit homolog 2 protein
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基因ID
- 9353
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NCBI登录号
- NP_004778
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UniProt
- O94813
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途径
- Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration
抗原
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