ATAD5 抗体 (AA 63-76)
Quick Overview for ATAD5 抗体 (AA 63-76) (ABIN401276)
抗原
See all ATAD5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 63-76
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特异性
- This antibody is directed against Elg1 protein.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- Synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
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亚型
- IgG
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anti-ATPase Family, AAA Domain Containing 5 (ATAD5) (Internal Region) antibody
Verified ATAD5 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-ATPase Family, AAA Domain Containing 5 (ATAD5) (AA 1151-1250) antibodyATAD5 适用: 小鼠 ELISA, WB, ICC, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ATPase Family, AAA Domain Containing 5 (ATAD5) (AA 63-76) antibodyATAD5 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: 1: 3,000 - 1: 15,000. Western blot: 1: 500 - 1: 2,000 (Expect a band approximately 120 kDa in size correspondingto Elg1 in the appropriate cell lysate or extract).
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限制
- 仅限研究用
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浓度
- 0.50 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))
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别名
- ATAD5
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背景
- ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.Synonyms: ATPase family AAA domain-containing protein 5, C17orf41, Chromosome fragility-associated gene 1 protein, FRAG1
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基因ID
- 79915
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NCBI登录号
- NP_079133
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UniProt
- Q96QE3
抗原
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